An More research is being done on the significance of volatile organic molecules for microbial function. However, because most studies have focused on volatiles produced by monocultures of well-described bacterial genera, little is now known about how inter-specific bacterial interactions influence volatiles production.We wanted to know how inter-specific bacterial interactions impacted the volatiles' composition, generation, and activity in this investigation. Chryseobacterium, Dyella and Janthinobacterium, four phylogenetically distinct bacterial species, were chosen. Previous research had demonstrated that, in contrast to monocultures, pairwise pairings of these bacteria exhibited antibacterial activity in agar media. In the present work, we investigated if the generation of antimicrobial volatiles similarly reflected these observations. As a result, both pairwise combinations and monocultures of the bacteria's volatiles were evaluated for identification and antibacterial activity. The volatiles' antimicrobial efficacy was evaluated using model bacterial, fungal, and oomycete species. Our findings showed that the composition of volatile blends was impacted by interactions between different bacterial species. In contrast, depending on the volatile blend composition, the effect of volatiles on bacteria varied from no effect to growth inhibition to growth encouragement. Fungi and oomycetes have shown considerable sensitivity to bacterial volatiles. A total of 35 volatile compounds were found, the majority of which contained sulphur. Dimethyl disulfide and dimethyl trisulfide, two frequently generated sulfur-containing volatile chemicals, were examined for their impacts on three target microorganisms. Here, we highlight the significance of interactions between different species for the formation of bacterial volatiles and their antibacterial properties.
